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S B Middleton1, I M Frayling2 and R K S Phillips11The Polyposis Registry, Imperial Cancer Research Fund Colorectal Cancer Unit, St Mark Hospital, Northwick Park, Watford Road, Harrow, Middlesex, HA1 3UJ, UK2Cambridge University Department of Medical Genetics, Molecular Genetics Laboratory, PO Box 158, Addenbrooke Hospital, Cambridge, CB2 2QQ, UKReceived 30 June 1999; Revised 4 October 1999; Accepted 6 October 1999Top of pageAbstractReferences Desmoids are poorly understood, locally aggressive, non metastasizing fibromatoses that occur with disproportionate frequency in patients with familial adenomatous polyposis (FAP). Their nature is controversial with arguments for and against a neoplastic origin. Neoplastic proliferations are by definition monoclonal, whereas reactive processes originate from a polyclonal background.
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Analysis of MeDIP seq data and identification of DMRsWe filtered out low quality reads that contained more than 5 ‘N’s and more than 50% of the sequences with a low quality value (Phred score17. The reads that mapped to multiple genomic locations were regarded as duplicates and were therefore selected only once. To avoid stochastic sampling drift, we filtered out CpG sites that were covered by less than a 10 read depth when performing subsequent analysis.